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Updated: Jun 25, 2026

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
Published on: January 17, 2025
[Biochemical methods for the analysis of DNA-protein interactions]
Jin-Bi Zhang1, Zeng-Xiang Pan, Fei Lin
1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. 2007105010@njau.edu.cn
Abstract:
Investigation of DNA-protein interactions is fundamental to understand the mechanism underlying a variety of life processes. In this article, various types of biochemical methods in DNA-protein interaction study in vivo and in vitro at the level of DNA, protein, and the complex, respectively were briefly reviewed. Traditional assays including Nitrocellulose filter-binding assay, Footprinting, EMSA, and Southwestern blotting were summarized. In addition, chromatin immunoprecipitation techniques including nChIP, xChIP, and ChIP-on-chip, which were widely used in epigenetics, were particularly introduced.
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