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Updated: Aug 14, 2026
07:44
Analysis of Fatty Acid Content and Composition in Microalgae
Published on: October 1, 2013
Quantitative recovery of free and esterified fatty acids from thin-layer plates coated with silica gel
Journal of Chromatography
|January 11, 1977
Abstract:
A method is described for the recovery of fatty acids from silica gel on thin-layer plates. The method is simple and rapid and depends on the conversion of silica gel to potassium silicate, using potassium hydroxide, followed by acidification in such a way that the silicic acid is kept in solution. Fatty acids are then extracted by shaking with solvent. Fatty acid recovery from free fatty acid, phospholipid, glyceride and cholesterol ester zones is more than 90% efficient. There is no oxidation or isomerization of unsaturated fatty acids.
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To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
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