Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neural Shape Modeling Reveals Early and Progressive Femoral Bone Shape and Cartilage Thickness Changes After Anterior Cruciate Ligament Reconstruction.

medRxiv : the preprint server for health sciences·2026
Same author

Protein Response to ACL Injury in Humans Show Early Cartilage Remodeling and Differences by Sex.

bioRxiv : the preprint server for biology·2026
Same author

Handheld Ultrasound for Personalized All-Soft-Tissue Quadriceps Tendon Anterior Cruciate Ligament Reconstruction.

Video journal of sports medicine·2026
Same author

BMI and Varus Malalignment Compound to Define a High-Risk Phenotype for Compartment-Specific Knee Osteoarthritis Progression.

medRxiv : the preprint server for health sciences·2026
Same author

What's New in Biologics.

The Journal of bone and joint surgery. American volume·2026
Same author

Adipose-derived stromal vascular fraction injection for radiation-induced bladder fibrosis: a first-in-human pilot study.

Stem cells translational medicine·2026
Same journal

Biomimetic Collagen Scaffolds Natural Cross-Linking Strategies via Transglutaminase and Methylglyoxal for Skin Repair.

Journal of biomedical materials research. Part A·2026
Same journal

Granular Hydrogel Composites for Noninvasive Optical Biosensing.

Journal of biomedical materials research. Part A·2026
Same journal

Performance Evaluation of Highly Uniform Astragalus Polysaccharide/Silk Fibroin Microspheres Fabricated via Microfluidics as Biodegradable Fillers: Collagen Regeneration and Tissue Reaction.

Journal of biomedical materials research. Part A·2026
Same journal

Combining Supramolecular and Covalent Chemistry to Form Reinforced Fibrillar Network Hydrogels From Fibrinogen Derivatives.

Journal of biomedical materials research. Part A·2026
Same journal

Bioactive-Loaded Detachable Crosslinked Hyaluronic Acid Microneedles: Structural Validation and Clinical Anti-Wrinkle Efficacy.

Journal of biomedical materials research. Part A·2026
Same journal

Tunable Sol-Gel Transition in Poloxamer Blends for Injectable Osteoarticular Applications.

Journal of biomedical materials research. Part A·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

Novel multiarm PEG-based hydrogels for tissue engineering.

Huaping Tan1, Alicia J DeFail, J Peter Rubin

  • 1Division of Plastic Surgery, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.

Journal of Biomedical Materials Research. Part A
|March 18, 2009
PubMed
Summary
This summary is machine-generated.

Injectable scaffolds made from poly(ethylene glycol) (PEG) hydrogels show promise for tissue engineering. Eight-arm PEG hydrogels offer slower gelation and better cell adhesion for regenerative medicine.

More Related Videos

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture
11:34

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Published on: December 26, 2017

Related Experiment Videos

Last Updated: Jun 24, 2026

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture
11:34

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Published on: December 26, 2017

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Polymer Chemistry

Background:

  • Injectable scaffolds are crucial for tissue engineering and regenerative medicine.
  • Poly(ethylene glycol) (PEG) hydrogels are widely explored for their biocompatibility and tunable properties.
  • Genipin, a natural crosslinker, offers a safer alternative to synthetic crosslinking agents.

Purpose of the Study:

  • To characterize multiarm amino-terminated PEG hydrogels crosslinked with genipin.
  • To evaluate the impact of PEG arm number on hydrogel properties and biological performance.
  • To assess the potential of these hydrogels as injectable cell carriers for tissue engineering.

Main Methods:

  • Synthesis and characterization of four- and eight-arm amino-terminated PEG hydrogels crosslinked with genipin.
  • Scanning electron microscopy (SEM) for structural analysis.
  • In vitro assessment of gelation time, water uptake, swelling, and weight loss.
  • In vitro cell culture studies using human adipose-derived stem cells (hADSCs).

Main Results:

  • Eight-arm PEG hydrogels exhibited significantly slower gelation times compared to four-arm PEG hydrogels.
  • Structural differences between four- and eight-arm PEG hydrogels influenced genipin crosslinking efficiency.
  • Human adipose-derived stem cells showed enhanced adhesion on four-arm PEG hydrogels versus eight-arm PEG hydrogels.
  • PEG hydrogels demonstrated suitable properties for in vitro cell carrier applications.

Conclusions:

  • Multiarm PEG hydrogels crosslinked with genipin are promising injectable scaffolds for regenerative medicine.
  • The number of PEG arms influences hydrogel kinetics and biological interactions.
  • Four-arm PEG hydrogels may offer advantages in cell adhesion for specific tissue engineering applications.