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Updated: Jun 24, 2026

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
Published on: March 30, 2018
Epstein-Barr virus as a possible etiologic agent in primary central nervous system lymphoma in immunocompetent
Ashwani Tandon1, Sundaram Challa, M Shanmugam
1Department of Pathology, Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad - 500 082. Andhra Pradesh, India. challa_sundaram@yahoo.com
Background:
Primary central nervous system lymphoma (PCNSL) occurs in both immunocompetent and immunosuppressed individuals. The role of Epstein-Barr virus (EBV) has been implicated in immunosuppressed individuals but its role is not established in immunocompetent individuals.
Aims:
To study the possible role of EBV in PCNSL in immunocompetent individuals.
Setting And Design:
Retrospective study.
Materials And Methods:
Thirty patients with PCNSL were studied immunohistochemically with antibodies to CD45, CD20, CD3 and EBV latent membrane protein-1 (EBV LMP-1). In situ hybridization was done in 19 patients where enough tissue was available using a specific oligonucleotide probe for EBV-Early RNA (EBER).
Results:
All the patients were immunocompetent and mean age was 41.6 years. Histologically they were diffuse large cell lymphoma: 25 (83.3%) were B cell, 1(3.3%) was T cell and 4 (13.3%) were unclassified. EBV LMP-1 showed variable membrane and cytoplasmic positivity in 24 (80%) patients. In situ hybridization for EBER was negative in all the 19 patients studied.
Conclusion:
In this region of the world probably EBV has no etiologic role in PCNSL in immunocompetent individuals.
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