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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

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Related Experiment Video

Updated: Jul 2, 2026

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

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Published on: November 29, 2018

Postmortem Sperm Retrieval in Hanging-Induced Mechanical Asphyxia: Probability and Temporal Dynamics.

Leena Lokhande¹, Raghvendra Kumar Vidua¹, Ashwani Tandon²

¹Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, India.

Journal of Human Reproductive Sciences

|April 10, 2026

Summary

This summary is machine-generated.

Viable sperm can be retrieved in 50% of hanging deaths up to 21.5 hours post-mortem. This study confirms the feasibility of post-mortem semen retrieval (PMSR) and challenges traditional sperm degradation timelines.

Keywords:

Hanging mechanical asphyxia motility post-mortem sperm retrieval semen quality time since death viability

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Last Updated: Jul 2, 2026

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Published on: November 29, 2018

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Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia

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Published on: May 27, 2022

Area of Science:

Forensic Medicine
Reproductive Biology
Toxicology

Background:

Post-mortem semen retrieval (PMSR) is crucial in sexual assault cases.
Understanding sperm viability post-mortem is vital for forensic investigations.

Purpose of the Study:

To assess the feasibility of retrieving viable spermatozoa in hanging deaths.
To correlate sperm quality with post-mortem interval (PMI) and other parameters.

Main Methods:

A descriptive observational study was conducted on 18 medicolegal autopsy cases.
Vaso-epididymal wash technique was used for sperm retrieval within 24 hours of death.
Sperm motility and viability were assessed using automated semen quality analyser and eosin dye exclusion test.

Main Results:

Motile sperm recovered in 44.4% and viable sperm in 50% of cases.
Mean motility and viability in positive samples were 14.5% and 29.9%, respectively.
Sperm viability persisted up to 21.5 hours post-mortem, with a peak viability of 72%.

Conclusions:

Post-mortem semen retrieval (PMSR) is feasible in hanging deaths, with viable sperm recoverable up to 21.5 hours.
Findings challenge linear sperm degradation models, suggesting motility loss precedes cell death.
Emphasizes the need for rapid processing and standardized medico-legal PMSR protocols in India.