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Updated: Feb 22, 2026

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
Published on: January 27, 2026
SUnSET, a nonradioactive method to monitor protein synthesis
Enrico K Schmidt1, Giovanna Clavarino, Maurizio Ceppi
1Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, France.
Abstract:
We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.

