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Updated: Jun 24, 2026
15:19
Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
[Construction and evaluation of a genetic engineered strain for biodesulfurization]
Huanjie Li1, Zhijian Yu, Xiaochao Xiong
1Laboratory of Biochemical and Molecular Biology, Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Summary
This study engineered a Pseudomonas delafieldii strain for enhanced biodesulfurization. The new strain significantly increased the removal of dibenzothiophene and sulfur from diesel fuel.
Area of Science:
- Microbial biotechnology
- Environmental microbiology
Context:
- Petroleum refining faces challenges with sulfur compounds.
- Biodesulfurization offers a greener alternative to traditional methods.
Purpose:
- To engineer a multi-copy desulfurization (dsz) operon strain of Pseudomonas delafieldii R-8.
- To enhance the efficiency of dibenzothiophene (DBT) biodegradation.
Summary:
- The dsz operon was cloned into a plasmid and reintroduced into Pseudomonas delafieldii R-8, creating strain R-8-1.
- Strain R-8-1 exhibited a two-fold increase in DBT removal rates compared to the wild-type.
- Application to diesel desulfurization resulted in a 68% sulfur reduction by strain R-8-1, versus 53% by the wild-type.
Impact:
- This work presents the first multi-copy dsz operon engineering strain.
- The enhanced strain shows promise for more efficient and sustainable biodesulfurization processes.
- The findings support further development in microbial fuel desulfurization technologies.
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