Application of polymerase chain reaction (PCR) to HLA-C locus typing
L I Sakkas1, R W Vaughan, G S Panayi
1Molecular Immunogenetics Unit, UMDS, Guy's Hospital, London, U.K.
Abstract:
Polymerase chain reaction/oligonucleotide typing was used to identify HLA-Cw*0601 (Cw6) in patients with psoriasis and psoriatic arthritis. The assignment of HLA-Cw*0601 was established by the concordant presence of codons for alanine (position 73), lysine (position 80) and tryptophan (position 97). The frequencies of all three codons were increased in the patient groups.
