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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Updated: Jun 24, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Gene regulation by methylation.

Wolf C Mueller1, Andreas von Deimling

  • 1Department of Neuropathology, Institute of Pathology, Im Neuenheimer Feld 220/221, Heidelberg 69120, Germany. Wolf.Mueller@med.uni-heidelberg.de

Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progres Dans Les Recherches Sur Le Cancer
|March 27, 2009
PubMed
Summary
This summary is machine-generated.

Epigenetic gene regulation, particularly O-6-methylguanine-DNA methyltransferase (MGMT) promoter methylation, impacts malignant glioma outcomes. While not currently guiding glioblastoma treatment, this molecular marker garners significant interest for potential therapeutic strategies.

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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Last Updated: Jun 24, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Epigenetic gene regulation significantly influences malignant glioma clinical outcomes.
  • O-6-methylguanine-DNA methyltransferase (MGMT) promoter methylation is the most studied epigenetic factor in glioblastoma.
  • Current treatment decisions for glioblastoma are not altered by MGMT promoter methylation analysis due to limited targeted therapies.

Purpose of the Study:

  • To provide an overview of epigenome characteristics in normal and malignant cells.
  • To discuss current methods for methylation analysis.
  • To explore future therapeutic targets for glioma.

Main Methods:

  • Review of physiological epigenome characteristics.
  • Analysis of epigenetic gene regulation changes in malignant transformation.
  • Discussion of technical aspects, advantages, and limitations of methylation analysis techniques.
  • Exploration of potential therapeutic strategies.

Main Results:

  • Aberrant promoter methylation can lead to tumor-suppressor gene silencing in gliomas.
  • Various methods exist for single-gene and genome-wide methylation analyses, each with pros and cons.
  • There is growing interest in assessing MGMT promoter methylation in glioblastoma patients.

Conclusions:

  • Understanding epigenetic alterations is crucial for glioma prognostication.
  • Advancements in methylation analysis offer insights into glioma biology.
  • Future therapeutic approaches may target aberrant epigenetic modifications to overcome gene silencing.