Related Experiment Video
Updated: Jun 24, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium
Sung-Jin Jo1, Chean Ring Leong, Ken'ichiro Matsumoto
1Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13 W8, Kita-ku, Sapporo-shi, Hokkaido, Japan.
Abstract:
We previously synthesized poly(3-hydroxybutyrate) [P(3HB)] in recombinant Corynebacterium glutamicum, a prominent producer of amino acids. In this study, a two-step cultivation was established for the dual production of glutamate and P(3HB) due to the differences in the optimal concentration of biotin. Glutamate was extracellularly produced first under the biotin-limited condition of 0.3 microg/L. Production was then shifted to P(3HB) by addition of biotin to a total concentration of 9 microg/L. The final products obtained were 18 g/L glutamate and 36 wt% of P(3HB).
Related Concept Videos
Bioreactor Controls-III
Production of Antibiotics
Production of Pharmaceuticals
Production of Organic Acids
Production of Alcohol
Upstream Processing

