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Growth inhibition of head and neck carcinomas by D-allose
Tomoo Mitani1, Hiroshi Hoshikawa, Terushige Mori
1Department of Otorhinolaryngology, Faculty of Medicine, Kagawa University, Kagawa, Japan. mitanit@med.kagawa-u.ac.jp
Background:
An inhibitory effect of D-allose, a rare sugar, on several cancer cell lines has been reported. This study aimed to investigate the growth inhibition of head and neck squamous cell carcinoma cells by D-allose.
Methods:
We treated 3 head and neck carcinoma cell lines with D-allose, D-fructose, D-psicose, and D-glucose. Cell growth assays as well as analyses of messenger RNA (mRNA) expression, cell cycle, apoptosis, and uptake of 14C-glucose were performed.
Results:
D-allose had inhibitory effects on all 3 cell lines and tended to upregulate mRNA expression of glucose transporters, p21 and p53, and downregulate mRNA expression of cyclin A2, cyclin B1, and CDC2. We observed that D-allose tended to interfere with the intracellular uptake of D-glucose and induced apoptosis.
Conclusion:
Our results indicate that D-allose inhibits the growth of head and neck squamous cell carcinoma cells. D-allose has a considerable potential as a new anticancer agent in those patients.
Insights
D-allose, a rare sugar, effectively inhibits head and neck squamous cell carcinoma growth. This study suggests D-allose shows promise as a novel anticancer agent for these patients.
Area of Science:
- Biochemistry
- Oncology
- Cell Biology
Background:
- D-allose, a rare sugar, has demonstrated inhibitory effects on various cancer cell lines.
- Head and neck squamous cell carcinoma (HNSCC) remains a significant health concern requiring novel therapeutic strategies.
Purpose of the Study:
- To investigate the potential of D-allose in inhibiting the growth of HNSCC cells.
- To elucidate the mechanisms underlying D-allose's anti-cancer effects in HNSCC.
Main Methods:
- Treatment of three HNSCC cell lines with D-allose, D-fructose, D-psicose, and D-glucose.
- Assessment of cell growth, mRNA expression (including glucose transporters, cell cycle regulators, and tumor suppressors), cell cycle progression, apoptosis, and 14C-glucose uptake.
Main Results:
- D-allose exhibited significant growth inhibition across all tested HNSCC cell lines.
- D-allose modulated mRNA expression of key genes, including upregulation of glucose transporters, p21, and p53, and downregulation of cyclin A2, cyclin B1, and CDC2.
- D-allose interfered with D-glucose uptake and induced apoptosis in HNSCC cells.
Conclusions:
- D-allose demonstrates potent inhibitory effects on HNSCC cell growth.
- D-allose presents considerable potential as a novel therapeutic agent for patients with head and neck squamous cell carcinoma.