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Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

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Cytokine multiplex analysis.

Howard A Young¹

¹Laboratory of Experimental Immunology, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 7, 2009

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Multiplex analysis allows simultaneous monitoring of multiple gene expressions, moving beyond single-gene studies in molecular and cell biology. This chapter reviews available assay systems for comprehensive gene expression profiling in research and clinical settings.

Area of Science:

Molecular Biology
Cell Biology
Systems Biology
Cancer Biology

Background:

Gene expression monitoring is crucial in biological research.
Systems biology necessitates analyzing multiple genes simultaneously, not just single genes.
Multiplex analysis enables the simultaneous measurement of 8-100 genes per sample.

Purpose of the Study:

To discuss commercially available assay systems for multiplex gene expression analysis.
To cover both RNA and protein analysis methods.
To provide an overview of current technologies in the field.

Main Methods:

Review of commercially available multiplex assay systems.
Discussion of technologies for simultaneous RNA expression analysis.

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Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

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Discussion of technologies for simultaneous protein expression analysis.

Main Results:

Various multiplex assay systems are commercially available for gene expression analysis.
These systems facilitate the routine monitoring of multiple RNA and protein targets.
The chapter provides a comprehensive overview of these technologies.

Conclusions:

Multiplex analysis is a standard technique in modern molecular and cell biology.
A range of commercial assay systems exist for multiplex RNA and protein analysis.
These technologies support systems biology approaches in research and clinical applications.