A new modified conducting carbon composite electrode as sensor for ascorbate and biosensor for glucose.

Area of Science:

• Electrochemistry
• Materials Science
• Biosensors

Background:

• Development of advanced materials for electrochemical sensing.
• Need for sensitive and selective detection of biomolecules like ascorbate and glucose.
• Limitations of existing carbon-based sensors.

Purpose of the Study:

• To develop a new carbon-based conducting composite for electrochemical sensing.
• To create a sensitive and selective sensor for ascorbate detection.
• To engineer an interference-free glucose biosensor with enhanced performance.

Main Methods:

• Fabrication of cellulose acetate-graphite composite electrodes.
• Electrocatalytic oxidation for ascorbate detection at unmodified electrodes.
• Immobilization of glucose oxidase on poly(neutral red) modified composite electrodes for glucose biosensing.

Main Results:

• Successful electrocatalytic oxidation of ascorbate with high sensitivity, selectivity, and a low detection limit at 0.0 V vs. SCE.
• Application of the ascorbate sensor for determination in commercial fruit juice samples.
• Development of an interference-free glucose biosensor with high sensitivity (31.5+/-1.7 microA cm(-2) mM(-1)), low detection limit (20.3 microM), and rapid response time.

Conclusions:

• The developed carbon-based conducting composite is effective for both electrochemical sensing of ascorbate and biosensing of glucose.
• The ascorbate sensor demonstrates practical utility in real-world food sample analysis.
• The glucose biosensor exhibits superior sensitivity and performance compared to other carbon-composite-based sensors.