Related Experiment Video
Updated: Jun 24, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
[Denitrifying bacteria isolated from river sediment and its nitrogen and phosphorus removal capacity from river
1Environmental and Healthy Engineering Research Center, Henan University, Kaifeng 475001, China. wlenglish@163.com
Abstract:
20 strains of bacteria were isolated from river sediment using enrichment culture medium for denitrification, and the denitrification intensity was determined. F10, one of bacteria strain, was identified having the highest denitrifying intensity, and further used to test its role in the removing of nitrogen and phosphorus from wastewater in laboratory. By checking the individual morphology, colony culture characteristics, DNA sequencing and 16S rDNA gene bank, F10 was identified as Alcaligenes faecalis, and its denitrifying intensity was 63.2%. The highest removal of TN (76.2%) and TP (93.8%) were observed in a 10 days period with an addition of F10 at a rate of 100 mg/L.
Related Concept Videos
Microbial Wastewater Treatment
Environmental Applications of Microorganisms
Metabolism of Chemolithotrophs
Microbes and the Nitrogen Cycle
Inorganic Nitrogen Assimilation
Microbial Bioremediation of Uranium

