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Updated: Jun 24, 2026
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Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography
Published on: December 27, 2024
A rapid HPLC method used to establish pediatric reference intervals for vitamins A and E
Kamisha L Johnson-Davis1, Steve J Moore, William E Owen
1Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84112, USA.
Summary
A new, rapid HPLC method accurately measures vitamin A and E levels in children. This method establishes crucial pediatric reference intervals for these essential fat-soluble vitamins.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Pediatric Nutrition
Background:
- Serum vitamin A (retinol) and E (tocopherol) levels are vital for assessing nutritional status and potential toxicity.
- Existing HPLC methods for vitamin analysis were time-consuming and resource-intensive.
Purpose of the Study:
- To develop and validate a faster, more efficient HPLC method for analyzing serum vitamins A and E.
- To establish reliable pediatric reference intervals for vitamins A and E.
Main Methods:
- Modified an existing HPLC method using high-throughput columns and small-diameter tubing.
- Serum proteins were precipitated, vitamins extracted into hexane, and analyzed via HPLC with UV detection.
Main Results:
- The modified HPLC method demonstrated strong correlation with the existing technique.
- Established age-dependent reference intervals for retinol and non-age-dependent intervals for retinyl palmitate, alpha-tocopherol, and gamma-tocopherol.
- Gender did not necessitate separate reference intervals.
Conclusions:
- Validated a rapid HPLC method, reducing run-time by 60% and reagent consumption.
- Successfully established pediatric reference intervals for vitamins A and E in 1136 healthy children (ages 7-17).