Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tunable electrostatic interactions of lipid-coated quantum dots with biological membranes.

bioRxiv : the preprint server for biology·2026
Same author

AlliGator: Open source fluorescence lifetime imaging analysis in G.

SoftwareX·2026
Same author

Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications.

PhotoniX·2025
Same author

Scattering-based super-resolution optical fluctuation imaging.

Optics express·2025
Same author

Toward measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime.

Biophysical reports·2025
Same author

Deep learning-based temporal deconvolution for photon time-of-flight distribution retrieval.

Optics letters·2024
Same journal

Intrinsic Superconducting Gap in Bilayer KCa<sub>2</sub>Fe<sub>4</sub>As<sub>4</sub>F<sub>2</sub> and Decoupled Monolayer FeAs.

Nano letters·2026
Same journal

Programmable Hydrogen-Assisted Chemical Vapor Deposition Growth and Bipolar Transport in Two-Dimensional MoO<sub>2</sub> Nanoflakes.

Nano letters·2026
Same journal

A Curvature-Modulated Strategy for Single-Atom Catalysts toward Reciprocal Regulation in Li-S Batteries.

Nano letters·2026
Same journal

Vacuum Pyrolysis Engineered CoSb/C Scaffold for Sodium Metal Anodes with Sodiophilic and Superionic Interphase.

Nano letters·2026
Same journal

Hexagonal SiGe Quantum Dots in Nanowires.

Nano letters·2026
Same journal

Monolithic Axial InGaAs Quantum Dot Emitters in GaAs-Based Nanowires via Sb-Mediated Facet Engineering.

Nano letters·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Nanometer distance measurements between multicolor quantum dots.

Josh Antelman1, Connie Wilking-Chang, Shimon Weiss

  • 1Department of Chemistry & Biochemistry, University of California at Los Angeles, Los Angles, California 90095, USA.

Nano Letters
|April 21, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a new multicolor imaging technique using quantum dots (QDs) to precisely measure distances in biomolecules. This method overcomes common imaging challenges, enabling accurate structural analysis of DNA and protein complexes.

More Related Videos

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Related Experiment Videos

Last Updated: Jun 23, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • Accurate structural determination of biomolecules is crucial for understanding their function.
  • Existing multicolor imaging techniques often suffer from chromatic aberrations and registration issues.
  • Quantum dots offer unique photophysical properties for advanced biological imaging.

Purpose of the Study:

  • To develop and validate a novel multicolor imaging method for precise distance measurements in biomolecular structures.
  • To address and overcome limitations of current multicolor imaging techniques, such as chromatic aberration.
  • To enable accurate nanometer-scale structural studies of biomolecules and their complexes.

Main Methods:

  • Fabrication of quantum dot dimers using DNA molecules labeled with different colored quantum dots.
  • Application of multicolor stage-scanning confocal microscopy for imaging.
  • Suppression of quantum dot blinking to enhance measurement accuracy.
  • Detailed characterization of factors contributing to measurement variability.

Main Results:

  • Demonstrated nanometer accuracy in individual distance measurements between quantum dots.
  • Successfully eliminated chromatic aberration and color registration issues inherent in other multicolor imaging methods.
  • Quantified the impact of various factors on measurement variability, ensuring reliability.
  • Validated the efficacy of the quantum dot dimer approach for structural analysis.

Conclusions:

  • The developed multicolor quantum dot imaging technique provides a robust platform for accurate biomolecular structural studies.
  • This method significantly improves upon existing techniques by eliminating optical aberrations.
  • The findings pave the way for advanced investigations into the structure and dynamics of biomolecular assemblies.