Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics
09:12

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Published on: May 28, 2016

Nanoparticles/Dip stick.

Yi Lu1, Juewen Liu, Debapriya Mazumdar

  • 1Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enzymes, DNAzymes and nanozymes for environmental remediation.

Nanoscale·2026
Same author

Combining G-Quadruplex and Non-Quadruplex Aptamers with Distinct Thermodynamic Driving Forces for Highly Selective Pb<sup>2+</sup> Detection.

ACS sensors·2026
Same author

Capture-SELEX derived aptamers for light-up fluorescent detection of carbendazim.

Talanta·2026
Same author

Affinity sensors for L-lactate and lactate dehydrogenase.

Analytical methods : advancing methods and applications·2026
Same author

Tuning Library Length Revealing High Affinity Melamine Aptamers with Opposite Fluorescence Responses for Highly Selective Detection.

Analytical chemistry·2026
Same author

SELEX-derived potassium aptamers for robust detection in a physiological sodium background.

Biosensors & bioelectronics·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Aptamer-linked gold nanoparticles (AuNPs) offer a novel colorimetric sensing method. These sensors disassemble upon target binding, enabling versatile detection and user-friendly dipstick tests.

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Aptamers are nucleic acid or peptide molecules that bind targets with high specificity.
  • Aptamer conformation changes upon analyte binding, enabling various sensing applications.
  • Traditional aptamer sensors require external signaling labels, often limiting their utility.

Purpose of the Study:

  • To develop aptamer-linked inorganic nanoparticles for analyte detection.
  • To create a colorimetric sensing platform utilizing nanoparticle disassembly.
  • To demonstrate the versatility and user-friendliness of the developed aptasensors.

Main Methods:

  • Preparation of aptamer-linked gold nanoparticles (AuNPs) designed for disassembly.
  • Utilizing the disassembly of AuNPs upon target analyte binding for colorimetric detection.

More Related Videos

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

Related Experiment Videos

Last Updated: Jun 23, 2026

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics
09:12

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Published on: May 28, 2016

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

  • Adapting the protocols for other inorganic nanoparticles like quantum dots and magnetic nanoparticles.
  • Integrating the aptasensor into lateral flow devices for dipstick testing.

    • Main Results:

    • Developed AuNPs that rapidly disassemble into dispersed nanoparticles upon target binding.
    • Demonstrated general applicability for colorimetric sensing of diverse analytes.
    • Successfully applied the method to quantum dots and magnetic nanoparticles.
    • Created user-friendly dipstick tests using lateral flow devices.

    Conclusions:

    • Aptamer-linked inorganic nanoparticles provide a robust platform for colorimetric sensing.
    • The described method is versatile, applicable to various nanoparticles and analytes.
    • The development of dipstick tests significantly enhances the practical usability of aptasensor technology.