Related Experiment Video
Updated: Jun 23, 2026
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
Reverse genetics of Nipah virus to probe viral pathogenicity
Misako Yoneda1, Kentaro Fujita, Hiroki Sato
1The University of Tokyo, Institute of Medical Science, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
Abstract:
Enhanced green fluorescent protein (EGFP) is a useful marker protein which enables the tracing of virus infection. Recombinant viruses expressing EGFP are useful for the investigation of the underlying mechanism of viral infection in vitro and in vivo. Using EGFP-expressing recombinant Nipah virus (NiV) and canine distemper virus (CDV), we tested the susceptibility of a variety of cells to infection. Receptor usage in CDV infection was also investigated.
