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Updated: Jun 23, 2026
07:44
Identifying Protein-protein Interaction Sites Using Peptide Arrays
Published on: November 18, 2014
Rapid identification of improved protein ligands using peptoid microarrays
Hyun-Suk Lim1, M Muralidhar Reddy, Xiangshu Xiao
1Department of Internal Medicine, Division of Translational Research, University of Texas Southwestern Medical Center, Dallas, TX 75390-9185, USA.
Bioorganic & Medicinal Chemistry Letters
|April 22, 2009
Abstract:
A rapid array-based protocol is presented by which a modest affinity protein-binding small molecule can be appended to a library of peptoids via click chemistry. The array can then be screened for improved ligands that exhibit a higher affinity for the protein target.
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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
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