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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Surface-enhanced Raman scattering: realization of localized surface plasmon resonance using unique substrates and
Mohammad Kamal Hossain1, Yasutaka Kitahama, Genin Gary Huang
1Department of Chemistry, School of Science and Technology, Kwansei Gakuin University, Gakuen 2-1, Sanda, Hyogo, 669-1337, Japan.
Analytical and Bioanalytical Chemistry
|April 23, 2009
Summary
This study explores gold and silver nanoparticle substrates for surface-enhanced Raman scattering (SERS). Elongated gold nanostructures demonstrate superior Raman signal enhancement, paving the way for sensitive biomolecule detection.
Area of Science:
- Plasmonics and Nanophotonics
- Spectroscopy
- Materials Science
Background:
- Surface-enhanced Raman scattering (SERS) relies on localized surface plasmon resonance (LSPR) for signal enhancement.
- The quality and reproducibility of SERS signals are critically dependent on the SERS substrate's properties.
- Diverse LSPR excitations at nanoparticle interfaces are key to achieving significant SERS enhancements.
Purpose of the Study:
- To develop novel gold and silver nanoparticle-based SERS substrates.
- To investigate the influence of LSPR excitations on SERS performance.
- To establish a correlation between LSPR characteristics and SERS signal enhancement.
Main Methods:
- Fabrication of surfactant-free gold nanoparticle monolayers (isolated aggregates, chain-like, 2D nanostructures) on glass slides.
- Characterization of localized surface plasmon resonance (LSPR) using spectroscopy.
- Simultaneous SERS measurements on the same spatial positions as LSPR analysis.
- Development of a novel approach for silver nanoparticle-based SERS detection of proteins and biomolecules in aqueous media.
Main Results:
- Elongated gold nanostructures exhibited higher SERS enhancement compared to isolated aggregates and 2D nanostructures.
- Distinct LSPR peak characteristics were observed for different gold nanoaggregate morphologies.
- A strong correlation was found between SPR and SERS images for gold nanoaggregates.
- A new strategy for aqueous biomolecule detection using silver SERS substrates was proposed.
Conclusions:
- The morphology of gold nanoaggregates significantly impacts LSPR and SERS enhancement.
- Surfactant-free gold substrates offer a promising platform for SERS applications.
- The developed silver nanoparticle strategy enables sensitive detection of biomolecules like glutathione.
- LSPR excitation is crucial for optimizing SERS performance in various applications.
