Related Experiment Video
Updated: Jun 23, 2026
Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
A high-performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of
Jody K Takemoto1, Neal M Davies
1College of Pharmacy Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program Washington State University, Pullman, WA 99164-6534, USA.
Abstract:
A high-performance liquid chromatographic method was developed for the analysis of 3'-hydroxypterostilbene. This method involves the use of a Luna C(18) column with ultraviolet detection at 325 nm. The mobile phase consisted of acetonitrile, water and formic acid (50:50:0.01, v/v/v) with a flow rate of 0.8 mL/min. The calibration curves were linear over the range 0.5-100.0 microg/mL. The mean extraction efficiency was between 97.40 and 111.16%. The precision of the assay was 0.196-14.39% (RSD%), and within 15% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <16% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and serum.
More Related Videos
05:03Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography
Published on: December 27, 2024
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013