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Updated: Jun 23, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
Development of high-throughput mass spectrometry-based approaches for cancer biomarker discovery and implementation
Brian L Hood1, Nicolas A S Stewart, Thomas P Conrads
1Clinical Proteomics Facility, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA.
Abstract:
A major goal of cancer research is elucidating the molecular events underlying carcinogenesis, with the goal of discovering better diagnostic markers and therapeutic targets. Proteomics aims to facilitate this process by applying newly developed methods and advanced analytic tools, such as mass spectrometry, for the investigation of the protein complement en masse. Proteomics is the comprehensive study of proteins and is aimed at analyzing their structure, function, modifications, expression, interactions, and localization in complex biological systems. This article reviews the state-of-the art in mass spectrometry-based approaches and their application for cancer biomarker discovery and validation.
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