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Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: Jun 23, 2026

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Fully dynamic multiple-beam optical tweezers.

Rene Eriksen, Vincent Daria, Jesper Gluckstad

    Optics Express
    |May 14, 2009
    PubMed
    Summary

    We developed a new method for creating dynamic optical tweezers using generalized phase contrast and a spatial light modulator. This technique allows for real-time control over multiple optical traps for precise manipulation.

    Area of Science:

    • Optics and Photonics
    • Biophysics
    • Microscopy and Imaging

    Background:

    • Optical tweezers are crucial tools for manipulating microscopic objects.
    • Existing methods for generating multiple optical traps can be complex and limited in dynamic control.

    Purpose of the Study:

    • To demonstrate a novel technique for generating fully dynamic multiple-beam optical tweezers.
    • To enable real-time, adaptable control over optical trap arrays.

    Main Methods:

    • Utilized the generalized phase contrast (GPC) method.
    • Employed a phase-only spatial light modulator (SLM) to shape optical beams.
    • Developed a straightforward process for transforming phase patterns into optical traps.

    Main Results:

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    Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo
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    Published on: November 2, 2018

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    Last Updated: Jun 23, 2026

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    Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

    Published on: October 13, 2011

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    Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

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    • Successfully generated an adjustable array of high-intensity beams acting as optical traps.
    • Achieved real-time control over the position, size, shape, and intensity of individual optical tweezers.
    • Demonstrated the trapping and dynamic manipulation of multiple micro-spheres in a liquid solution.

    Conclusions:

    • The GPC method combined with an SLM offers a flexible and efficient approach for creating dynamic multiple-beam optical tweezers.
    • This technique provides precise control over optical trap arrays, advancing capabilities in micro-manipulation.