The thickness of electrospun poly (epsilon-caprolactone) nanofibrous scaffolds influences cell proliferation

Nanofibrous scaffolds have morphological similarities to native extracellular matrix and have been considered as candidate scaffolds in tissue engineering. However, there is no report on the effect of the thickness of nanofibrous scaffold on cell behavior. In this study poly (epsilon-caprolactone) (PCL) nanofibrous scaffolds with thicknesses of 0.1 and 0.6 mm were fabricated by electrospinning. Properties of PCL nanofibrous scaffolds were measured by contact angle and air permeability measurements while the morphology of the nanofibers was observed by SEM. Mouse embryonal carcinoma stem cells (P19), monkey epithelial kidney cells (Vero), Chinese hamster ovary cells (CHO) and mouse mesenchymal stem cells (MSCs) were seeded on PCL nanofibrous scaffolds with thicknesses of 0.1 and 0.6 mm. Air permeability measurements showed that air permeability decreases with the increase in the thickness of nanofibrous scaffolds, and contact angle measurements revealed a contact angle of 118 degrees for electrospun PCL nanofibers. The MTT assays showed that the proliferation of the cells was influenced by the thickness of the nanofibrous scaffold. Scaffolds with a thickness of 0.6 mm were found to provide a better substrate for cell proliferation, possibly due to more dimensional stability. Therefore, regardless of cell origin, thicker scaffolds provide a better substrate for cell proliferation, possibly due to the higher dimensional stability and tightness of thicker scaffolds.