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Updated: Jun 23, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

Electrospun nanostructured scaffolds for bone tissue engineering.

Molamma P Prabhakaran¹, J Venugopal, S Ramakrishna

¹Nanoscience and Nanotechnology Initiative, Division of Bioengineering, National University of Singapore, Singapore 117576, Singapore. biempp@nus.edu.sg

Acta Biomaterialia

|May 19, 2009

Summary

This summary is machine-generated.

This study developed a novel poly-l-lactide/collagen/nanohydroxyapatite nanofibrous scaffold for bone tissue engineering. The scaffold enhanced osteoblast proliferation and mineralization, showing potential for bone regeneration.

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Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Bone tissue engineering aims to create bioartificial bone grafts that mimic the extracellular matrix (ECM) for effective bone regeneration.
Biocomposite polymeric nanofibers incorporating nanohydroxyapatite (HA) are promising scaffolds for bone regeneration.
Current challenges include achieving effective bone mineralization and promoting osteoblast activity.

Purpose of the Study:

To fabricate and characterize novel nanofibrous scaffolds for bone tissue engineering.
To evaluate the in vitro biocompatibility and osteogenic potential of poly-l-lactide (PLLA), PLLA/HA, and PLLA/collagen/HA nanofibrous scaffolds.
To investigate the synergistic effects of collagen and HA on osteoblast behavior and mineralization.

Main Methods:

Fabrication of PLLA, PLLA/HA, and PLLA/collagen/HA nanofibrous scaffolds using electrospinning.
Morphological, chemical, and mechanical characterization using scanning electron microscopy, Fourier transform infrared spectroscopy, and tensile testing.
In vitro assessment of human fetal osteoblast (hFOB) proliferation, alkaline phosphatase activity (ALP), and mineralization.

Main Results:

PLLA/collagen/HA nanofibers demonstrated enhanced osteoblast adhesion, proliferation, and mineralization compared to PLLA and PLLA/HA scaffolds.
Mineral deposition was 57% higher on PLLA/collagen/HA nanofibers than on PLLA/HA nanofibers.
The combination of collagen and HA in PLLA nanofibers provided cell recognition sites and promoted osteoconduction.

Conclusions:

The PLLA/collagen/HA nanofibrous scaffold is a promising substrate for osteoblast proliferation and mineralization.
The synergistic effect of collagen and HA enhances the osteogenic potential of the scaffold.
This biocomposite scaffold holds potential for improving bone regeneration in tissue engineering applications.