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Updated: Jun 23, 2026

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
Published on: January 19, 2020
Visualization of membrane loss during the shrinkage of giant vesicles under electropulsation
Thomas Portet1, Franc Camps i Febrer, Jean-Michel Escoffre
1Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, UMR 5089, Université Paul Sabatier, Toulouse, France.
Abstract:
We study the effect of permeabilizing electric fields applied to two different types of giant unilamellar vesicles, the first formed from EggPC lipids and the second formed from DOPC lipids. Experiments on vesicles of both lipid types show a decrease in vesicle radius, which is interpreted as being due to lipid loss during the permeabilization process. We show that the decrease in size can be qualitatively explained as a loss of lipid area, which is proportional to the area of the vesicle that is permeabilized. Three possible modes of membrane loss were directly observed: pore formation, vesicle formation, and tubule formation.

