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Direct detection and quantification of microRNAs

Eric A Hunt1, Ann M Goulding, Sapna K Deo

  • 1Department of Chemistry & Chemical Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USA.

Analytical Biochemistry
|May 21, 2009
PubMed
Summary

No abstract available in PubMed .

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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...