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Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
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[Molecular-genetic comparison of Streptococcus pyogenes strains types emm1 and emm12 on prophage genes repertoire]

E M Poliakova, V Gao, Y Yang

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |May 23, 2009
    PubMed
    Abstract

    Insights

    Molecular analysis of Streptococcus pyogenes emm1 and emm12 strains revealed shared prophage genes. This suggests similar bacteriophages may circulate among different Group A Streptococcus strains.

    Area of Science:

    • Microbiology
    • Genomics
    • Molecular Biology

    Background:

    • Streptococcus pyogenes (Group A Streptococcus) is a significant human pathogen.
    • Specific emm types, such as emm1 and emm12, are associated with distinct clinical manifestations.
    • Prophage DNA integrated into bacterial genomes can influence virulence and host adaptation.

    Purpose of the Study:

    • To investigate the presence and distribution of prophage genes within emm1 and emm12 strains of Streptococcus pyogenes.
    • To compare the prophage gene content between emm1 and emm12 types to identify similarities and differences.

    Main Methods:

    • Genomic DNA was extracted from 31 S. pyogenes emm1 and 22 S. pyogenes emm12 strains.
    • Polymerase chain reaction (PCR) was employed using specific primers to detect 24 different phage-associated genes.

    Main Results:

    • The prevalence of specific phage genes, including speA, speC, and ssa in emm1 strains, and speC and speH in emm12 strains, was determined.
    • Nineteen distinct prophage gene profiles were identified across the analyzed strains.
    • Significant overlap in prophage gene content was observed between emm1 and emm12 strains.

    Conclusions:

    • The study identified both shared and distinct prophage gene repertoires between emm1 and emm12 Streptococcus pyogenes strains.
    • The presence of common prophage genes suggests the potential circulation of similar or identical bacteriophages among different emm types of Group A Streptococcus in the studied region.
    • Understanding prophage-host interactions is crucial for deciphering bacterial evolution and pathogenicity.

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