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Updated: Jun 23, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

Published on: September 11, 2016

[Aseptic meningitis season caused by enteroviruses in 2007: molecular-epidemiological aspects]

G G Onishchenko, N A Novikova, E I Efimov

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |May 23, 2009
    PubMed
    Abstract

    Insights

    Aseptic meningitis (AM) cases surged in Nizhny Novgorod due to enterovirus E30. Molecular analysis revealed this E30 strain was linked to European variants, suggesting a potential transmission pathway.

    Area of Science:

    • Virology
    • Epidemiology
    • Molecular Biology

    Context:

    • Aseptic meningitis (AM) is a significant public health concern, often caused by enteroviruses.
    • Understanding the drivers of AM outbreaks is crucial for effective public health interventions.
    • Nizhny Novgorod experienced a notable increase in AM cases between 2001 and 2007.

    Purpose:

    • To analyze the epidemic patterns of aseptic meningitis in Nizhny Novgorod.
    • To identify the causative agents and their molecular characteristics during the outbreak.
    • To investigate the potential sources and transmission routes of enteroviruses responsible for AM.

    Summary:

    • Enteroviruses were identified as the primary cause of a marked rise in aseptic meningitis (AM) in Nizhny Novgorod from 2001 to 2007.
    • During a peak in August-October 2007, enteroviruses were detected in 93.8% of AM patients, with serotype E30 being the dominant strain (53.1%).
    • The dominant E30 subtype (E30-N1) showed genetic similarity to European strains, while another subtype (E30-N2) was related to Asian strains. Viral RNA in nasopharyngeal swabs suggests aspiration as a transmission route.

    Impact:

    • Identified a specific enterovirus variant (E30) as the cause of the 2007 AM outbreak.
    • Provided insights into the genetic relatedness of the causative agent to international strains, aiding in understanding viral evolution and spread.
    • Highlighted the role of nasopharyngeal shedding in the rapid transmission of enteroviruses causing AM.

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