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Related Experiment Video

Updated: Jun 23, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Single-shot phase-stepped wide-field coherencegated imaging.

Christopher Dunsby, Y Gu, P French

|May 23, 2009

Summary

This study introduces a novel, cost-effective coherence-gated imaging method using a single CCD camera for simultaneous phase-stepped image acquisition. The technique enables real-time, depth-resolved imaging of dynamic and scattering samples.

Area of Science:

Optics and Photonics
Biomedical Imaging
Digital Image Processing

Background:

Coherence-gated imaging (CGI) offers depth-resolved visualization but often requires complex setups.
Traditional phase-stepping techniques typically necessitate multiple acquisitions or specialized hardware.
Dynamic biological and material samples present challenges for imaging due to motion artifacts.

Purpose of the Study:

To develop a simplified, single-shot wide-field coherence-gated imaging system.
To enable simultaneous acquisition of four phase-stepped images using a single CCD camera.
To provide a cost-effective solution for real-time, depth-resolved imaging of dynamic samples.

Main Methods:

A novel optical configuration utilizing spatially separated phase-stepped images.

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Last Updated: Jun 23, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph

Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph

Published on: February 21, 2025

Implementation of a single, wide-field Charge-Coupled Device (CCD) camera for simultaneous data capture.

Phase-stepping interferometry adapted for single-shot acquisition.

Main Results:

Demonstration of real-time coherence-gated imaging of a moving watch cog.
Successful 3D reconstructions of a coin.
Accurate phase measurements of a test-chart surface.
Depth-resolved imaging achieved in a weakly scattering onion sample.

Conclusions:

The presented technique offers a simplified and economical approach to coherence-gated imaging.
Simultaneous acquisition of phase-stepped images overcomes limitations of dynamic sample imaging.
The system's versatility is shown through diverse imaging applications.