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Updated: Jun 22, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
In vitro toxicity of MTA compared with other primary teeth pulpotomy agents
José Vitor Nogara Borges de Menezes1, Esther Rieko Takamori, Maria Francisca Thereza Borro Bijella
1Pediatric Dentistry, Universidade Federal do Paraná, Curitiba, Brazil. jvmenezes@gmail.com
Objective:
The main goal of this work is to compare the In vitro toxicity of MTA with other primary teeth pulpotomy agents.
Study Design:
The In vitro toxicity of MTA, calcium hydroxide, ferric sulphate solution, diluted formocresol and Buckley's formocresol were tested using MTT and Neutral Red Uptake cell viability assays. The results for MTA were compared to those obtained for the other substances using ANOVA and Tukey statistical tests (p < 0.05).
Results:
MTA had the lower in vitro toxicity and Buckley's formocresol, the higher, with statically significant difference.
Conclusion:
Among the primary teeth pulpotomy agents tested, MTA showed the lower In vitro toxicity, standing as the most promising substitute to formocresol.