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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Published on: October 18, 2016
Possible role for cellular FXIII in monocyte-derived dendritic cell motility
Asier Jayo1, Isabel Conde, Pedro Lastres
1Department of Cellular and Molecular Medicine, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain.
European Journal of Cell Biology
|May 30, 2009
Summary
Cellular factor XIII (FXIII-A) regulates cell movement. Upregulation of FXIII-A in dendritic cells enhances their migration, suggesting a key role in cell motility.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Plasma factor XIII A subunit (FXIII-A) is a transglutaminase involved in intracellular processes like cytoskeleton remodeling.
- Its specific function within monocytes and macrophages, and the impact of its absence, are not fully understood.
Purpose of the Study:
- To investigate the role of cellular FXIII (cFXIII) in monocyte-derived dendritic cell (DC) migration.
- To determine the functional consequences of cFXIII expression on cell motility.
Main Methods:
- Monocytes were differentiated into dendritic cells (DCs) in vitro.
- Transglutaminase activity was inhibited using monodansyl-cadaverine.
- Migration assays (transwell, scratch-wound) were performed on wild-type and FXIII-A-deficient DCs, as well as on CHO cells expressing FXIII-A.
- Cellular FXIII (cFXIII) expression levels were analyzed during DC differentiation.
Main Results:
- cFXIII expression increased during monocyte to DC differentiation.
- Inhibition of transglutaminase activity reduced DC migration.
- FXIII-A-deficient DCs exhibited impaired chemotaxis towards CCL19.
- CHO cells expressing FXIII-A demonstrated enhanced motility and increased membrane blebbing.
Conclusions:
- Upregulation of cFXIII in dendritic cells plays a significant role in regulating cell motility.
- FXIII-A contributes to DC migration and the formation of membrane protrusions essential for cell movement.
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