Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler.

Theresa Caragine¹, Rebecca Mikulasovich, Jeannie Tamariz

¹Department of Forensic Biology, Office of Chief Medical Examiner of the City of New York, 421 East 26th Street, New York, NY 10016, USA. tcaragine@ocme.nyc.gov

Croatian Medical Journal

|May 30, 2009

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Small pieces make the big picture: the etiology of the relationship between executive functions and personality traits.

BMC psychology·2025

Same author

Personality and <i>COMT</i> gene: molecular-genetic and epigenetic associations with NEO-PI-R personality domains and facets in monozygotic twins.

Frontiers in genetics·2024

Same author

Introduction to the JFS Special Section on Disaster Victim Identification (DVI).

Journal of forensic sciences·2024

Same author

Global survey on evaluative reporting on DNA evidence with regard to activity-level propositions.

Journal of forensic sciences·2024

Same author

Development of Improved DNA Collection and Extraction Methods for Handled Documents.

Genes·2023

Same author

Differences in <i>MB-COMT</i> DNA methylation in monozygotic twins on phenotypic indicators of impulsivity.

Frontiers in genetics·2023

Short tandem repeat (STR) profiling of low template DNA (LT-DNA) is reliable and robust when using defined testing and interpretation parameters. This validated method, with quality control, is suitable for forensic casework.

Area of Science:

Forensic Science
Molecular Biology
Genetics

Background:

Low template DNA (LT-DNA) presents challenges in forensic analysis due to stochastic effects.
Standardized testing and interpretation parameters are crucial for reliable LT-DNA profiling.

Purpose of the Study:

To evaluate the reliability, robustness, and reproducibility of short tandem repeat (STR) profiling for LT-DNA.
To establish and validate defined testing and interpretation parameters for LT-DNA analysis.

Main Methods:

Quantitative real-time PCR was used to measure DNA down to less than 1 pg/µL.
DNA extracts were amplified in triplicate using enhanced PCR conditions and analyzed independently.
Interpretation protocols were developed considering stochastic effects and validated on over 800 samples.

More Related Videos

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

Related Experiment Videos

Last Updated: Jun 22, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

Main Results:

Reproducible results were obtained for DNA amounts of 100 pg or less, with anticipated stochastic effects.
Allele detection rates were 92% or higher down to 25 pg, with lower amounts yielding partial profiles.
100% accurate allelic assignments were achieved for over 400 casework samples using the developed protocols.

Conclusions:

The established protocols and interpretation guidelines demonstrate that LT-DNA testing is reliable and robust for forensic applications.
Implementation of verified methods with quality control ensures the suitability of LT-DNA testing in forensic science.