Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LY-CoV1404 (bebtelovimab) potently neutralizes SARS-CoV-2 variants.

Cell reports·2022
Same author

LY-CoV1404 (bebtelovimab) potently neutralizes SARS-CoV-2 variants.

bioRxiv : the preprint server for biology·2021
Same author

Cultivating DNA Sequencing Technology After the Human Genome Project.

Annual review of genomics and human genetics·2020
Same author

Rapid and highly-specific generation of targeted DNA sequencing libraries enabled by linking capture probes with universal primers.

PloS one·2018
Same author

Duplex Proximity Sequencing (Pro-Seq): A method to improve DNA sequencing accuracy without the cost of molecular barcoding redundancy.

PloS one·2018
Same author

Somatic mutation detection using various targeted detection assays in paired samples of circulating tumor DNA, primary tumor and metastases from patients undergoing resection of colorectal liver metastases.

Molecular oncology·2017
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
08:50

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Published on: May 12, 2023

Nanopore force spectroscopy on DNA duplexes.

Nahid N Jetha1, Matthew Wiggin, Andre Marziali

  • 1Department of Physics and Astronomy, University of British Columbia, V6T 1Z1, Vancouver, BC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2009
PubMed
Summary
This summary is machine-generated.

Nanopore force spectroscopy measures DNA duplex dissociation times under applied force. This technique distinguishes between perfect and mismatched DNA sequences, enabling sensitive detection.

More Related Videos

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Related Experiment Videos

Last Updated: Jun 22, 2026

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
08:50

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Published on: May 12, 2023

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Force spectroscopy offers a method to study charged molecules like nucleic acids.
  • Nanopore technology allows for the investigation of molecular interactions and dynamics at the single-molecule level.

Purpose of the Study:

  • To present a detailed protocol for nanopore force spectroscopy of DNA duplexes.
  • To measure DNA duplex dissociation times under applied force.
  • To demonstrate the potential for sequence detection using nanopore force spectroscopy.

Main Methods:

  • Utilizing single-stranded DNA (ssDNA) probes anchored and threaded through alpha-hemolysin nanopores.
  • Applying electric potential to exert force on the ssDNA, promoting duplex translocation.
  • Measuring duplex dissociation times as a function of applied force.

Main Results:

  • Dissociation time scales for perfectly complementary DNA duplexes differ significantly (over two orders of magnitude) from those with a single mismatch.
  • The technique allows for the measurement of duplex survival probability under force.
  • Characteristic dissociation time scales can be determined from recorded dissociation events.

Conclusions:

  • Nanopore force spectroscopy is a viable technique for studying DNA duplex stability and binding energies.
  • The method provides a sensitive approach for detecting sequence variations in DNA.
  • This technique holds promise for applications in molecular diagnostics and sequence analysis.