Related Experiment Video
Updated: Jun 22, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Published on: August 5, 2008
Comparative genomic hybridization on spotted oligonucleotide microarrays
Young H Kim1, Jonathan R Pollack
1Department of Pathology, Stanford University, Stanford, CA, USA.
This study presents a validated protocol for array-based comparative genomic hybridization (Array-CGH) using minimal DNA. The method offers high-resolution detection of genomic copy number alterations in various sample types.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- DNA microarray technology enables detailed genomic characterization.
- Array-based comparative genomic hybridization (Array-CGH) detects DNA copy number alterations.
- High-resolution genomic analysis is crucial for understanding complex genomes.
Purpose of the Study:
- To present a validated protocol for Array-CGH.
- To enable Array-CGH with minimal genomic DNA.
- To achieve high spatial resolution and reproducibility.
Main Methods:
- Utilizing print-tip spotted Human Exonic Evidence Based Oligonucleotide (HEEBO) microarrays.
- Employing as little as 25 ng of genomic DNA.
- Applying the protocol to diverse sources like cell lines and clinical specimens.
Main Results:
- The protocol is validated for Array-CGH.
- High spatial resolution in detecting copy number alterations is achieved.
- High array-to-array reproducibility is demonstrated.
Conclusions:
- The presented Array-CGH protocol is effective and reproducible.
- This method facilitates detailed genomic characterization with minimal DNA input.
- The protocol is suitable for various biological samples, including clinical specimens.
More Related Videos
09:16Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
Published on: February 21, 2015
09:32An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
Published on: November 8, 2017
Related Concept Videos
DNA Microarrays
FISH - Fluorescent In-situ Hybridization
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
In-situ Hybridization
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...