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Updated: Jun 22, 2026

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Published on: January 7, 2016
Analysis of major isoforms of human growth hormone before and after intensive physical exercise
D A Sakharov1, M Thevis, A G Tonevitsky
1Russian Physical Training and Sports Institute, Moscow. dimitri_sakharov@mail.ru
Abstract:
We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90% circulating growth hormone. It was demonstrated that the proportion of growth hormone isoforms in human serum before and after strenuous exercise remained unchanged.
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