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Pseudobasophilia and the Advia 120
Graham Gibbs1, Gill Campbell, Iain Christie
1Department of Haematology, Musgrove Park Hospital, Taunton, Somerset, TA1 5DA, UK. graham.gibbs@tst.nhs.uk
Hematology (Amsterdam, Netherlands)
|June 4, 2009
Summary
Pseudobasophilia, an analyzer artifact where abnormal cells are counted as basophils, can be identified using Advia 120 cytogram analysis. This method helps alert scientists to unusual peripheral blood cell populations.
Area of Science:
- Hematology
- Clinical Laboratory Science
- Medical Technology
Background:
- Pseudobasophilia is an artifactual finding in automated hematology analyzers.
- It occurs when abnormal peripheral blood cells are misclassified as basophils.
- This phenomenon has been previously noted on Technicon H-series analyzers.
Purpose of the Study:
- To describe pseudobasophilia as a technical consideration on the Advia 120 hematology analyzer.
- To explain how understanding the basophil method and cytogram can identify abnormal cell populations.
- To assist laboratory scientists and clinicians using the Advia 120 and Advia 2120 analyzers.
Main Methods:
- Analysis of the basophil (baso) method on the Advia 120.
- Interpretation of the baso cytogram generated by the Advia 120.
- Routine laboratory practice incorporating cytogram analysis.
Main Results:
- Pseudobasophilia is a relevant technical consideration for the Advia 120 analyzer.
- Advia 120 cytogram analysis can detect the presence of abnormal peripheral blood cells.
- This analysis serves as an alert for laboratory scientists and clinicians.
Conclusions:
- Understanding the Advia 120's basophil method and cytogram is crucial for accurate analysis.
- Cytogram analysis should be integrated into routine laboratory practice.
- This approach helps identify potential abnormal cell populations, aiding clinical diagnosis.
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