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Related Experiment Video

Updated: Jun 22, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Compatible pollinations in Solanum chacoense decrease both S-RNase and S-RNase mRNA.

Bolin Liu1, David Morse, Mario Cappadocia

  • 1Institut de Recherche en Biologie Végétale (IRBV), Biology Department, University of Montreal, Montreal, Québec, Canada.

Plos One
|June 4, 2009
PubMed
Summary
This summary is machine-generated.

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Plants prevent self-fertilization using gametophytic self-incompatibility (GSI). This study reveals compatible pollen signals to styles, reducing S-RNase levels and reinforcing successful fertilization.

Area of Science:

  • Plant reproduction
  • Molecular biology
  • Genetics

Background:

  • Gametophytic self-incompatibility (GSI) prevents self-fertilization in plants by blocking pollen with matching S-alleles.
  • Stylar S-RNase is crucial for GSI in Solanum chacoense, targeting incompatible pollen tubes.
  • Mechanisms enabling compatible pollen to avoid S-RNase are not fully understood.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying compatible pollen's evasion of S-RNase.
  • To identify signaling pathways involved in successful fertilization during GSI.

Main Methods:

  • Comparative analysis of S-RNase levels in styles during compatible and incompatible crosses.
  • Quantification of pollen-specific Lat52 mRNA levels.
  • Assessment of S-RNase transcript levels in response to pollen signaling.

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Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Related Experiment Videos

Last Updated: Jun 22, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Main Results:

  • Compatible crosses showed up to 30% reduction in non-self stylar S-RNase compared to incompatible crosses.
  • No degradation of self S-RNases was observed.
  • Compatible pollen significantly reduced S-RNase transcript levels in transmitting tract cells, suggesting a feedback loop.

Conclusions:

  • A reciprocal signaling mechanism exists between compatible pollen and the female reproductive tract.
  • This feedback loop, involving decreased S-RNase transcript levels, may reinforce successful fertilization.
  • The findings uncover a novel regulatory pathway in plant self-incompatibility.