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Updated: Jun 22, 2026

12:58
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Published on: September 12, 2019
Topography and recognition imaging of protein-patterned surfaces generated by AFM nanolithography
Rong Zhu1, Andreas Ebner, Markus Kastner
1Institut für Biophysik, Johannes Kepler Universität, Altenberger Str. 69, 4040 Linz, Austria.
Abstract:
Native-protein nanolithography is combined with topography and recognition imaging to synergistically use AFM tips to write and image nanoscale protein patterns on a surface (see picture). The approach is validated with different feedback modes, using surface-bound biotinylated bovine serum albumin (BSA) protein and AFM tips carrying streptavidin.

