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Related Concept Videos

Interference and Diffraction02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

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Related Experiment Video

Updated: Jun 22, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Digital holography with a point diffraction interferometer.

C Iemmi, A Moreno, J Campos

    Optics Express
    |June 5, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed a digital holography technique using a point diffraction interferometer and liquid crystal television for 3D microscopy. This method works under partially coherent light, offering new possibilities for biological imaging.

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    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Digital Imaging

    Background:

    • Digital holography enables 3D reconstruction of microscopic objects.
    • Point diffraction interferometry is a sensitive technique for wavefront measurement.
    • Liquid crystal television displays offer reconfigurable optical elements.

    Purpose of the Study:

    • To propose and validate a novel digital holography technique.
    • To adapt point diffraction interferometry for 3D microscopy applications.
    • To utilize a liquid crystal television display for holographic implementation.

    Main Methods:

    • Implementation of a point diffraction interferometer.
    • Operation under partially coherent illumination.
    • Utilizing a liquid crystal television display as a spatial light modulator.

    Main Results:

    • Successful generation of digital holograms.
    • Demonstration of 3D imaging capabilities.
    • Validation of the theoretical framework.

    Conclusions:

    • The proposed technique is effective for 3D microscopy.
    • Partially coherent illumination is suitable for this holographic method.
    • The use of LCTV enhances the practicality of digital holography.