Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
09:45

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Published on: June 12, 2018

Directing polyallylamine adsorption on microlens array patterned silicon for microarray fabrication.

Gaurav Saini1, Richard Gates, Matthew C Asplund

  • 1Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.

Lab on a Chip
|June 5, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Customizable 3D-Printed Neural Probe Array Using Two-Photon Polymerization with Millimeter-Scaled Conductive Polymer as an Electrode Material.

ACS applied materials & interfaces·2026
Same author

Assessing cancer risk from pesticide exposure in selected rural areas of Greater Noida.

Environmental monitoring and assessment·2026
Same author

From Flat to 3D: Replacing Phenyl Rings with Saturated Bridged Bicyclic Mimetics to Mitigate Bioactivation and Optimize ADME Properties and Oral Bioavailability in Reverse Amide Orientation.

Journal of medicinal chemistry·2026
Same author

Retraction notice to "Trace element pollution tracking in the complex multi-aquifer groundwater system of Al-Hassa Oasis (Saudi Arabia) using spatial, chemometric and index-based techniques" [Environ. Res. 249 (2024) 118320].

Environmental research·2026
Same author

Respiratory deposition of particulate matter in Delhi: a five-year assessment of exposure patterns and health risks.

Scientific reports·2025
Same author

Cadmium and Chromium exposure associated with DNA hypermethylation of p16 tumor suppressor gene: a case-control study from endemic region of northern India.

Clinical epigenetics·2025
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

Researchers developed a method to selectively immobilize polyallylamine (PAAm) onto specific spots on silicon chips for bioarrays. A cationic surfactant was key to preventing nonspecific adsorption on hydrophobic surfaces, enabling targeted reactions.

Area of Science:

  • Surface chemistry
  • Materials science
  • Biotechnology

Background:

  • Selective reagent adsorption is crucial for bioarray and lab-on-a-chip devices.
  • Photopatterning of alkyl monolayers on silicon shards creates thousands of wells for targeted applications.

Purpose of the Study:

  • To develop a method for selective adsorption of polyallylamine (PAAm) onto patterned silicon surfaces.
  • To enhance the functionality of bioarrays by controlling polymer deposition.

Main Methods:

  • Photopatterning of silicon shards using a microlens array.
  • Deposition of polyallylamine (PAAm) with and without cationic surfactants (cetyltrimethylammonium chloride).
  • Characterization using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), X-ray Photoelectron Spectroscopy (XPS), spectroscopic ellipsometry, and wetting experiments.

More Related Videos

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

Polymeric Microneedle Array Fabrication by Photolithography
08:15

Polymeric Microneedle Array Fabrication by Photolithography

Published on: November 17, 2015

Related Experiment Videos

Last Updated: Jun 22, 2026

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
09:45

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Published on: June 12, 2018

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

Polymeric Microneedle Array Fabrication by Photolithography
08:15

Polymeric Microneedle Array Fabrication by Photolithography

Published on: November 17, 2015

Main Results:

  • Polyallylamine (PAAm) showed poor selectivity on hydrophobic monolayers without additives.
  • Addition of a cationic surfactant (cetyltrimethylammonium chloride) effectively prevented nonspecific PAAm adsorption on hydrophobic areas.
  • PAAm was successfully directed to specific spots, enabling localized bioconjugate chemistry reactions with glutaric anhydride, phenylenediisothiocyanate, biotin NHS ester, and DNA.

Conclusions:

  • Cationic surfactants are effective in achieving selective polyallylamine (PAAm) adsorption on patterned silicon surfaces.
  • This method enables the creation of functionalized bioarrays with high spatial control.
  • The findings are significant for the development of advanced lab-on-a-chip devices and bioanalytical tools.