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Related Concept Videos

Urinary Bladder01:23

Urinary Bladder

The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
Anatomy of the Genitourinary System II: Bladder and Urethra01:19

Anatomy of the Genitourinary System II: Bladder and Urethra

The lower urinary system consists of the urinary bladder and urethra, which are essential in storing and expelling urine from the body. Together with the internal and external sphincters, these structures work together to regulate urination effectively.Anatomy of the BladderThe urinary bladder is a muscular, stretchable organ behind the pubic bone and in front of the rectum. In females, the bladder is positioned anterior to the vagina and inferior to the uterus, while in males, it is located...

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Related Experiment Video

Updated: Jun 22, 2026

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
10:19

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Published on: August 9, 2012

Tissue engineering in urinary bladder: morphological and functional characterization.

Danilo Zani1, Claudio Simeone, Nicola Arrighi

  • 1Divisione Clinicizzata di Urologia, Università degli Studi di Brescia, Italy. dazani@tiscali.it

Archivio Italiano Di Urologia, Andrologia : Organo Ufficiale [Di] Societa Italiana Di Ecografia Urologica E Nefrologica
|June 9, 2009
PubMed
Summary
This summary is machine-generated.

Tissue engineering offers potential for urology reconstructive surgery. Cultivated urothelial and detrusorial cells maintain original tissue characteristics in vitro, showing promise for future applications.

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Area of Science:

  • Urology
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tissue engineering is increasingly explored for urological therapies requiring reconstructive surgery.
  • This study investigates the potential of cultivated urothelial and detrusorial tissues for reconstructive applications.

Purpose of the Study:

  • To evaluate the morphological and functional aspects of cultured urothelial and detrusorial tissues.
  • To assess the feasibility of using these engineered tissues in reconstructive urology.

Main Methods:

  • Obtained urothelial and detrusorial cell cultures from animal (pigs, rabbits) and human sources.
  • Validated urothelial cell nature using Hematoxylin-Eosin staining, immunofluorescence (cytokeratin antibodies), and immunohistochemistry (cytokeratins 7, 17, 20).
  • Characterized detrusorial tissue using alpha-actin specific antibodies.

Main Results:

  • Isolated urothelial and smooth muscle cells retained original tissue characteristics in vitro.
  • These characteristics were preserved in both monolayer cultures and on scaffolds.
  • In vitro findings indicate the possibility of generating bladder tissue with properties similar to the native organ.

Conclusions:

  • Cultivated urothelial and detrusorial cells show promise for urological tissue engineering.
  • Further in vitro and in vivo investigations are necessary to explore clinical applications.
  • Engineered vesical tissues hold potential for future reconstructive surgery in urology.