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[The functional activity of the macrophages in experimental intestinal dysbacteriosis in rats]
Abstract:
The study of the functional activity of macrophages in the peritoneal exudate and spleen of Sprague-Dawley rats was made under the conditions of dysbacteriosis caused by the administration of antibiotics (gentamicin and phenoxymethylpenicillin) and short-term food deprivation. The complex study of the key functions of macrophages, carried out on the same pool of cells, showed that under the conditions of antibiotic therapy the enhanced function of ingestion was accompanied by a decrease in all other investigated functional parameters of peritoneal macrophages: the intensity of protein synthesis, oxidation metabolism, the activity of lysosomal apparatus, 5'-nucleotide activity. In contrast to changes observed in macrophages after the administration of antibiotics, short-term food deprivation induced increased functional activity as shown by most tests used in this investigation (such as tests for the ingestion of sheep red blood cells, 51Cr, nitro blue tetrazolium, acridine orange, 5'-nucleotidase activity), but did not affect the intensity of protein synthesis in macrophages. The comparison of these results with the data obtained by the authors after the administration of an immunomodulating agent made it possible to regard short-term food deprivation, judging by its effect on the functional activity on macrophages, as an immunostimulating action.
Insights
Antibiotics impaired macrophage function, reducing protein synthesis and metabolism. Short-term food deprivation, however, boosted macrophage activity, suggesting an immunostimulating effect.
Area of Science:
- Immunology
- Cell Biology
- Microbiology
Background:
- Dysbacteriosis, an imbalance in gut microbiota, can affect immune cell function.
- Antibiotics and dietary interventions are known to influence host-microbe interactions and immune responses.
Purpose of the Study:
- To investigate the impact of antibiotic-induced dysbacteriosis and short-term food deprivation on macrophage functional activity.
- To compare the effects of these two interventions on key macrophage functions.
Main Methods:
- Functional assessment of peritoneal macrophages from Sprague-Dawley rats.
- Evaluation of macrophage functions including phagocytosis, protein synthesis, oxidative metabolism, lysosomal activity, and enzyme activity (5'-nucleotidase).
- Comparison of effects induced by gentamicin/phenoxymethylpenicillin versus short-term food deprivation.
Main Results:
- Antibiotic administration led to enhanced phagocytosis but decreased protein synthesis, oxidative metabolism, lysosomal activity, and 5'-nucleotidase activity in macrophages.
- Short-term food deprivation increased macrophage phagocytosis and most other functional parameters, except protein synthesis.
- Macrophage functional changes induced by food deprivation were comparable to those of known immunostimulating agents.
Conclusions:
- Antibiotic-induced dysbacteriosis negatively impacts multiple macrophage functions.
- Short-term food deprivation exhibits an immunostimulating effect on macrophage functional activity.
- Macrophage functional assays provide insights into the immunomodulatory effects of dietary interventions.