Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

miR-124-3p mediates polygenic risk shared between schizophrenia and bipolar disorder.

Neuron·2023
Same author

Suicidal ideation and behavior in youth in low- and middle-income countries: A brief review of risk factors and implications for prevention.

Frontiers in psychiatry·2022
Same author

Early-life stress lastingly impacts microglial transcriptome and function under basal and immune-challenged conditions.

Translational psychiatry·2022
Same author

Association of Attention-Deficit/Hyperactivity Disorder and Depression Polygenic Scores with Lithium Response: A Consortium for Lithium Genetics Study.

Complex psychiatry·2022
Same author

Transcriptomic Studies of Antidepressant Action in Rodent Models of Depression: A First Meta-Analysis.

International journal of molecular sciences·2022
Same author

Meta-analysis of epigenome-wide association studies of major depressive disorder.

Scientific reports·2022

Related Experiment Video

Updated: Jun 22, 2026

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

A quantitative GC-MS method for three major polyamines in postmortem brain cortex.

Gary G Chen1, Gustavo Turecki, Orval A Mamer

  • 1McGill Group for Suicide Studies, Douglas Hospital, McGill University, Montreal, Quebec, Canada.

Journal of Mass Spectrometry : JMS
|June 11, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a fast, quantitative method for measuring polyamines (putrescine, spermidine, spermine) in postmortem brain tissue. This technique aids research into suicide epidemiology by analyzing these key metabolites.

More Related Videos

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
07:08

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

A Chromatin Assay for Human Brain Tissue
11:31

A Chromatin Assay for Human Brain Tissue

Published on: March 21, 2008

Related Experiment Videos

Last Updated: Jun 22, 2026

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
07:08

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

A Chromatin Assay for Human Brain Tissue
11:31

A Chromatin Assay for Human Brain Tissue

Published on: March 21, 2008

Area of Science:

  • Neurochemistry
  • Analytical Chemistry
  • Forensic Science

Background:

  • Polyamines like putrescine, spermidine, and spermine are critical ornithine metabolites in mammals.
  • Altered polyamine levels are implicated in various neurological conditions and may correlate with suicide risk.

Purpose of the Study:

  • To develop and validate a novel, rapid, and robust quantitative method for analyzing putrescine, spermidine, and spermine in human postmortem brain tissue.
  • To support epidemiological studies investigating the relationship between polyamine concentrations and suicide frequency.

Main Methods:

  • A novel extractive derivatization using ethylchloroformate and trifluoroacetylation for sample preparation.
  • Quantitative analysis via gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring.
  • Utilized stable isotope-labeled and chemical analogs as internal standards for accurate quantification.

Main Results:

  • Achieved sensitive quantification limits: 10 ng/g for putrescine, 100 ng/g for spermidine, and 1000 ng/g for spermine in wet brain tissue.
  • The method is significantly faster and less labor-intensive than previous techniques.
  • Demonstrated robustness for reliable analysis in postmortem samples.

Conclusions:

  • The developed GC-MS method offers a simple, rapid, and reliable approach for quantifying key polyamines in postmortem human brain.
  • This methodology provides a valuable tool for future epidemiological research, particularly in understanding the biochemical correlates of suicide.