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Related Experiment Video

Updated: Jun 22, 2026

Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
08:25

Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response

Published on: January 25, 2014

High-throughput quantification of root growth using a novel image-analysis tool.

Andrew French1, Susana Ubeda-Tomás, Tara J Holman

  • 1Centre for Plant Integrative Biology, University of Nottingham, Nottingham LE12 5RD, United Kingdom. andrew.french@cpib.ac.uk

Plant Physiology
|June 12, 2009
PubMed
Summary

This study introduces RootTrace, an automated method for tracking plant root growth dynamics. This high-throughput tool quantifies root length, curvature, and gravitropism, aiding plant development research.

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Area of Science:

  • Plant Biology
  • Developmental Biology
  • Bioinformatics

Background:

  • Understanding plant growth dynamics is crucial for plant development research.
  • Automated, high-throughput methods are needed to analyze complex root system architectures.
  • Quantifying root growth parameters like length, curvature, and stimulus response is essential.

Purpose of the Study:

  • To develop and validate a high-throughput, automated method for tracing Arabidopsis seedling roots.
  • To enable quantitative analysis of root growth dynamics, including gravitropism and curvature.
  • To provide an open-source tool for researchers studying plant root development.

Main Methods:

  • A novel method combining a particle-filtering algorithm with a graph-based approach to trace root center lines.

More Related Videos

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data
09:37

Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data

Published on: April 26, 2016

Related Experiment Videos

Last Updated: Jun 22, 2026

Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
08:25

Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response

Published on: January 25, 2014

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data
09:37

Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data

Published on: April 26, 2016

  • A top-down imaging approach robust to noise and flexible across different image sets.
  • Development of the RootTrace software for automated analysis of time-lapse root growth sequences.

    • Main Results:

    • The RootTrace tool successfully traced Arabidopsis seedling roots grown on agarose plates.
    • Quantitative analysis of root length, curvature, and gravitropic response was achieved.
    • The method demonstrated robustness to noise and flexibility across different image datasets.

    Conclusions:

    • RootTrace provides an efficient, automated, and user-friendly tool for plant root growth analysis.
    • The open-source availability of RootTrace facilitates further research in plant development and phenotyping.
    • This method significantly advances the ability to study root dynamics and stimulus responses in plants.