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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Chromatographic Resolution01:15

Chromatographic Resolution

In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Related Experiment Video

Updated: Jun 22, 2026

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
05:45

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Published on: June 8, 2021

Chiral separations by CE employing CDs.

Salvatore Fanali1

  • 1Institute of Chemical Methodologies, National Council of Research, Area della Ricerca di Roma I, Monterotondo Scalo (Rome), Italy. salvatore.fanali@imc.cnr.it

Electrophoresis
|June 12, 2009
PubMed
Summary

This study reviews 25 years of using cyclodextrins (CDs) in capillary electrophoresis (CE) for chiral separations. It highlights the significant role of CDs in resolving optical isomers through inclusion complexation.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Electromigration methods, particularly capillary electrophoresis (CE), are crucial for separating optical isomers.
  • Chiral selectors, especially cyclodextrins (CDs) and their derivatives, are vital tools in enantioseparation.
  • The development of CE for enantioseparation has seen significant advancements over the past 25 years.

Observation:

  • This paper traces the historical development of chiral separations using electromigration with CDs.
  • It details various enantioresolution mechanisms and the application of numerous chiral selectors in CE.
  • The significant role of inclusion-complexation with CDs in CE for chiral separations is emphasized.

Findings:

  • The authors' group has been instrumental in optimizing methods for enantiomer resolution using CD chiral selectors.

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Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
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Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

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Related Experiment Videos

Last Updated: Jun 22, 2026

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
05:45

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Published on: June 8, 2021

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
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Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

Published on: August 18, 2017

  • A key publication from their group served as a foundation for subsequent advancements in the field.
  • Key achievements include the development of novel chiral selectors, theoretical understanding, new methodological approaches, and diverse practical applications.

    • Implications:

    • The research underscores the enduring importance and versatility of CE with CDs as a technique for chiral separations.
    • It provides a historical perspective and highlights the cumulative progress in enantioselective separations.
    • The findings pave the way for future innovations in chiral selector design and separation methodologies.