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Updated: Jun 22, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Melting of DNA double strand after binding to geroprotective tetrapeptide
V Kh Khavinson1, A Yu Solovyov, L K Shataeva
1St. Petersburg Institute of Bioregulation and Gerontology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia.
Abstract:
Experimental relationship between the hyperchromic effect of DNA [poly(dA-dT):poly(dA-dT)] interacting with Ala-Glu-Asp-Gly peptide is presented by a saturation isotherm. The free DNA double strand is melting (the strands separate) at 69.5 degrees C and at higher energy expenditures (enthalpy increase by 976.4 kJ/mol b.p.) in comparison with melting of the DNA-peptide complex (28 degrees C and 444.6 kJ/mol b.p.). The detected regularities of melting of duplex DNA and the thermodynamic parameters of this process indicate the natural mechanism of interaction between DNA and regulatory peptides underlying functioning of the living matter.
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