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Published on: February 20, 2015
Occurrence and genotypes of Giardia isolated from lambs in Spain
María Teresa Gómez-Muñoz1, Cristina Navarro, María Magdalena Garijo-Toledo
1Departamento de Producción Animal, Sanidad Animal y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad CEU Cardenal Herrera, Avda. Seminario S/N 46113 Moncada, Valencia, Spain. mtgomez@uch.ceu.es
Abstract:
Three hundred and eighty six faecal specimens were randomly collected from 1- to 3-month-old lambs from 16 farms in Spain to investigate the presence of different genotypes of Giardia duodenalis. Individual specimens were examined by IFA (Immunofluorescence assay) and beta-giardin PCR polymerase chain reaction. Cysts of G. duodenalis were shed by lambs in every flock analyzed, showing a prevalence by farms of 100%. The average prevalence of G. duodenalis for the 386 specimens was 42%, ranging from 8.3 to 80% depending on the farm. beta-giardin PCR positive samples were sequenced to determine the genotypes present at each farm and seven new subtypes of beta-giardin Assemblage E are reported in this study. In each farm, one to six different beta-giardin subtypes were found, showing the high variability of the target. Also, one flock had the zoonotic Assemblage A. This is the first report of Giardia subgenotype A-1 in sheep in Spain.
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