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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: Jun 22, 2026

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
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Published on: February 21, 2017

Implementation of a fast method for high resolution phase contrast tomography.

A Groso, R Abela, M Stampanoni

    Optics Express
    |June 17, 2009
    PubMed
    Summary

    This study introduces a novel method for 3D phase imaging of weakly absorbing objects using a single tomographic dataset. The technique minimizes radiation dose while maintaining high-quality reconstructions for effective object analysis.

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    Published on: October 24, 2019

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    Last Updated: Jun 22, 2026

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
    10:18

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

    Published on: February 21, 2017

    3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
    07:01

    3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

    Published on: October 24, 2019

    Area of Science:

    • Physics
    • Optical Imaging
    • Medical Imaging

    Background:

    • Phase imaging provides valuable information about transparent objects.
    • Conventional methods often require multiple datasets, increasing radiation exposure.
    • Absorption artifacts can degrade the quality of phase reconstruction.

    Purpose of the Study:

    • To develop a single-dataset method for 3D phase (refractive index) distribution reconstruction.
    • To reduce absorption artifacts in phase imaging.
    • To minimize radiation dose in tomographic imaging.

    Main Methods:

    • Implementation of an amended Bronnikov algorithm for phase reconstruction.
    • Inclusion of a semi-empirically derived factor in the filter to reduce absorption artifacts.
    • Validation using an established phase contrast technique.

    Main Results:

    • Successful 3D phase distribution reconstruction from a single tomographic dataset.
    • Reconstruction quality sufficient for segmentation and postprocessing, despite assuming constant absorption.
    • Spatial resolution comparable to conventional absorption-based techniques.
    • Demonstrated performance through validation with established phase contrast methods.

    Conclusions:

    • The developed method offers a one-step approach for 3D phase imaging.
    • It effectively minimizes radiation dose while yielding high-quality reconstructions.
    • The technique is suitable for analyzing weakly absorbing objects in various scientific fields.