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Related Experiment Video

Updated: Jun 22, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

F Gu1, Y Li, C Zhou

  • 1Division of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, California 90095, USA.

The Open Dentistry Journal
|June 23, 2009
PubMed
Summary
This summary is machine-generated.

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The liquid phase of human saliva contains bacterial nucleic acids, reflecting the oral microbiota. This finding suggests the cell-free saliva supernatant provides insights into oral bacteria composition.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • Human saliva separates into cellular and liquid phases via centrifugation.
  • The cellular phase is known to harbor oral bacteria, but the liquid phase's microbial content is poorly understood.

Purpose of the Study:

  • To investigate the presence and correlation of bacterial nucleic acids in the cell-free liquid phase of human saliva.
  • To determine if the liquid phase reflects the oral microbiota composition found in the cellular phase.

Main Methods:

  • Analysis of bacterial 16S ribosomal DNA (rDNA) and 16S ribosomal RNA (rRNA) in saliva liquid phase using universal bacterial primers.
  • Polymerase chain reaction (PCR) amplification and sequencing of 16S rDNA from the liquid phase.
  • Denaturing gradient gel electrophoresis (DGGE) to compare microbial profiles between liquid and cellular phases.
Keywords:
16S rDNA16S rRNADGGE.Salivaoral bacteria

Related Experiment Videos

Last Updated: Jun 22, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
12:33

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

Main Results:

  • Significant amounts of bacterial 16S rRNA and rDNA were detected in the cell-free saliva liquid phase.
  • Sequencing identified 15 operational taxonomic units (OTUs) from the liquid phase amplicons.
  • DGGE analysis revealed a strong positive correlation (Pearson Correlation=0.822, P<0.001) between microbial profiles of the liquid and cellular phases.

Conclusions:

  • The liquid phase of human saliva contains abundant bacterial nucleic acids.
  • These nucleic acids in the liquid phase correlate with the bacteria present in the cellular phase of saliva.
  • The cell-free saliva supernatant can serve as a source of information about the oral microbiota.